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MB Sample ID: SA183927
Local Sample ID: | 32D_T4_I3_G1_L1 |
Subject ID: | SU002028 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley |
Age Or Age Range: | 8 weeks |
Weight Or Weight Range: | 250-450 g |
Gender: | Male and female |
Animal Animal Supplier: | Charles River |
Animal Light Cycle: | 12 h reverse light-dark cycles |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
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Sample Preparation:
Sampleprep ID: | SP002034 |
Sampleprep Summary: | A standard spiked IPA solution was prepared with 250 µL of SPLASH II Lipidomix and 14.750 mL of IPA. Serum samples were thawed on ice for one hour prior to the addition of the IPA solution in a 1:3 v/v ratio to separate lipids and small non-polar metabolites from proteins. Mixtures of serum and IPA solution were vortexed for 10 s and centrifuged at 16000g for 7 min. The supernatant was then collected for LC-MS analysis. Sample blanks were prepared with 50 µL of LC-MS grade water, and pooled QC samples were prepared from 5 µL aliquots of all study subject serum samples. Serum reference samples from uninjured Sprague-Dawley rat serum were processed in the same manner as study subject serum samples. |