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MB Sample ID: SA193717
Local Sample ID: | 2021_06_16_Jorida_Run 11 |
Subject ID: | SU002136 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002142 |
Sampleprep Summary: | Lipids were extracted from the cell pellet (Bligh/Dyer). 900 ul chlorofrom/methanol (1/2) into the cell pellet, then incubated at 4C for 1 hour agigated (300rpm). Chloroform(300ul) and MQ water(250ul) were added discrete order, then vortex and cetrifuged for 2min at 9000 rpm. Bottom organic phase was collected and dried using vacuum concentrator. |