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MB Sample ID: SA220538
Local Sample ID: | P37_V4 |
Subject ID: | SU002382 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002388 |
Sampleprep Summary: | Serum samples were prepared using the Agilent Bravo metabolomics platform. This automated platform was programmed to pipette 100 µL of serum into a 96-well plate from a 96-well plate that contained 200 µL of serum. Following this, 450 μL of a 1:1 mixture of methanol:ethanol was added to the plate to precipitate proteins. The supernatant was then applied to a Captiva EMR-lipid 96-well plate and left to stand for 5 min and a vacuum was applied to the plate (2-5 Hg) to initiate flow. Eluents were collected into 1 mL 96-well plates and subsequently dried under a stream of nitrogen. Prior to analysis, samples were reconstituted with 100 µL of 10 % methanol and were agitated on a plate shaker (MTS 2/4m IKA) at 600 rpm for 10 min. |