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MB Sample ID: SA245691
Local Sample ID: | SP_FFA_7 |
Subject ID: | SU002544 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 6 weeks |
Gender: | Female |
Animal Animal Supplier: | JAX |
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Sample Preparation:
Sampleprep ID: | SP002550 |
Sampleprep Summary: | Tissues were mechanically homogenized with the Precellys 24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France) in methanol containing internal standards (500 pmol/sample of d4-labeled EPA, d5-labeled PGE2, LPC with a 17:0 fatty acyl chain (LPC17:0), and PC with two 14:0 fatty acyl chains (PE14:0-14:0)) and then incubated overnight at -20°C. For extraction of phospholipids and lysophospholipids, one-tenth of tissue lysates were added to 10 volumes of 20 mM Tris-HCl (pH 7.4) and were extracted using the method of Bligh and Dyer. For extraction of oxygenated fatty acid metabolites, nine-tenths of the tissue lysates were added to water (final methanol concentration of 10% (v/v)), and the lipids were extracted using an Oasis HLB cartridge (Waters, Milford, MA, USA). |