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MB Sample ID: SA254477
Local Sample ID: | KIND2 |
Subject ID: | SU002622 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
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Sample Preparation:
Sampleprep ID: | SP002628 |
Sampleprep Summary: | Lipids were extracted according to MTBE method. Briefly, a 200-µL volume of water was added to 30 mg sample and vortexed for 5 s. Subsequently, 240 µL of precooling methanol was added and the mixture vortexed for 30 s. After that, 800 µL of MTBE was added and the mixture was ultrasound 20 min at 4℃ followed by sitting still for 30 min at room temperature. The solution was centrifuged at 14000g for 15min at 10℃ and the upper organic solvent layer was obtained and dried under nitrogen. |