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MB Sample ID: SA288789
Local Sample ID: | KO3_003 |
Subject ID: | SU002846 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 26 weeks |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP002852 |
Sampleprep Summary: | A coronal slice of brain (approximate 15 mg) at (-0.88 mm to -1.335mm from Bregma) plane was sectioned and homogenized in cold Methanol/Water (80/20, v/v) to a final concentration of 30mg/ml. Following 20 min of gentle sonication in Bioruptor (30 s on, 30 s off, 20 cycles) at 4 °C, samples were centrifuged for 10 min at 10,000xg at 4 °C and were processed for LC-MS/MS |