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MB Sample ID: SA294307
Local Sample ID: | 22 |
Subject ID: | SU002877 |
Subject Type: | Bacteria |
Subject Species: | Bacillus pumilus |
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Sample Preparation:
Sampleprep ID: | SP002883 |
Sampleprep Summary: | The broth cultures were centrifuged at 7000 rpm for 10 min under 4 °C and the supernatants were collected. Ethyl acetate (EtOAc) with 2 × volume was added and sonicated for 10 min. The samples were then centrifuged at 7000 rpm at 4 °C for 10 min and the organic phase was collected and dried using a rotary evaporator. The extracts were resuspended in 1:1 acetonitrile: H2O with 1% formic acid. These samples were diluted 20 times using methanol. All samples were filtrated through 0.22 µm syringe filters. |