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MB Sample ID: SA300480
Local Sample ID: | T6_118DR |
Subject ID: | SU002904 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002910 |
Sampleprep Summary: | Amniotic fluid metabolites were extracted by addition of 1 part amniotic fluid to 15 parts 70% acetonitrile in ddH2O (vol:vol). The mixture was briefly vortexed and then centrifuged for 5 min at 16,000 × g to pellet precipitated proteins. The protein pellet was solubilized in 0.2M NaOH and quantified by DC Protein Assay (Bio-Rad). The volume of metabolite extract was normalized by amniotic protein content. |