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MB Sample ID: SA309405
Local Sample ID: | A_1 |
Subject ID: | SU002974 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002980 |
Sampleprep Summary: | For fecal samples, 60mg of the sample was mixed with 600μL of 80% iced methanol, which included 0.001mg/mL 4-chloro-DL-phenylalanine as an internal standard. Zirconia crushing beads were added to the mixture, and the samples were disrupted using a vibratory crusher operating at 60 hertz. Subsequently, the samples were processed by ultrasonication in an ice bath for 10 minutes and left to stand at -20℃ for 30 minutes. The supernatants were collected by centrifugation at 20,000 g under 4 C for 10 minutes and then mixed with three-fold volumes of distilled water. After ultrasonication for 3 minutes to ensure even mixing, the samples were placed in a -20℃ environment for 2 hours. Finally, the supernatants obtained from centrifugation were transferred to sample vials for metabolomics analysis |