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MB Sample ID: SA323810
Local Sample ID: | 7 |
Subject ID: | SU003097 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP003103 |
Sampleprep Summary: | Frozen serum samples of Cohort-1 participants, together with internal standard compounds were sonicated in ice-cold methanol (500 μL), to which an equal volume of chloroform and 0.4 times the volume of ultrapure water (LC/MS grade, Wako Pure Chemical, Tokyo, Japan) were added. The suspension was centrifuged at 15,000 × g for 15 min at 4 °C. The aqueous phase was then filtered in an ultrafiltration tube (Ultrafree MC-PLHCC, Human Metabolome Technologies, Tsuruoka City, Japan), and the filtrate was concentrated using a vacuum concentrator (SpeedVac; Thermo Fisher Scientific, Waltham, MA, USA). The concentrated filtrate was dissolved in 50 μL ultrapure water and subjected to IC-HR-MS analysis. Methionine sulfone (L-Met) and 2-morpholinoethanesulfonic acid (MES) were used as internal standards for cationic and anionic metabolites, respectively. |