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MB Sample ID: SA326864
Local Sample ID: | BAT_6C_chow_neg_9 |
Subject ID: | SU003120 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP003126 |
Sampleprep Summary: | For metabolite extraction, tissues were weighed and then homogenized with extraction buffer which consisted of 80% methanol containing Phenylalanine-D₈ internal standard at a 40:1 volume to wet weight ratio. Samples were then centrifuged at 16,000 x g at 4 °C for 15 min. Finally, 50 μL supernatant was transferred to the glass insert for LC-MS detection. |