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MB Sample ID: SA333283
Local Sample ID: | 02_002 |
Subject ID: | SU003208 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Weight Or Weight Range: | 400g |
Gender: | Male and female |
Animal Animal Supplier: | Charles River |
Animal Housing: | Double Housed |
Animal Light Cycle: | Reverse Light Cycle |
Species Group: | Sprague Dawley |
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Sample Preparation:
Sampleprep ID: | SP003214 |
Sampleprep Summary: | Experimental batch 1 and 2 aliquoted tissue samples were thawed simultane-ously on ice prior to addition of solvent (IPA and Splash II Lipidomix in (1:3 v/v)) to sep-arate lipids and small non-polar metabolites. LC-MS grade water was used to prepare sample blanks, and pooled quality control (QC) samples were prepared from 5 µL ali-quoted supernatant of all samples in the study. The brain and solvent (1:4 w/v), and beads were placed in a Tissuelyser II for 8 min and centrifuged at 16000 g for 7 min. The supernatant was collected for LC-MS. Pooled quality control samples were formed from combining 6 µL aliquots of all brain sample extracts. Sample blanks were prepared with the same procedure except instead of a brain sample, 50 µL of LC-MS grade water was used. |