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MB Sample ID: SA165030
Local Sample ID: | 12h ATP_5 |
Subject ID: | SU001856 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | Human Lung epithelial A549 cancer cells |
Cell Passage Number: | 10 |
Cell Counts: | 5 million cells |
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Treatment:
Treatment ID: | TR001869 |
Treatment Summary: | 5 million A549 cells were treated with control (no treatment), 0.5 mM ATP or 10 ng/ml TGF-beta for 2, 6 and 12 hours. |
Treatment: | In vitro treatment with small molecules |
Treatment Compound: | 0.5 mM ATP and 10 ng/ml TGF-beta |
Treatment Dosevolume: | 10 ml DMEM media containing appropriate compounds |
Cell Storage: | 37C; 5% CO2 incubator |
Cell Growth Container: | 150 mm Dish tissue culture treated |
Cell Media: | DMEM (10% FBS; 1% Pen/Strep) |
Cell Harvesting: | after 2, 6 and 12 hours |
Cell Pct Confluence: | 80% |
Cell Media Lastchanged: | NA |