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MB Sample ID: SA245792
Local Sample ID: | bivalves_21C_C125_07 |
Subject ID: | SU002545 |
Subject Type: | Invertebrate |
Subject Species: | Ruditapes philippinarum |
Taxonomy ID: | 129788 |
Height Or Height Range: | length: 3.81 ± 0.42 cm; and width: 3.06 ± 0.51 cm |
Gender: | Male and female |
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Treatment:
Treatment ID: | TR002557 |
Treatment Summary: | Sea warming and 17-α-ethinylestradiol exposure. After depuration, the organisms were subjected to a chronic toxicity test for 28 days, consisting of exposure to five different EE2 concentrations: 0 (control group), 5, 25, 125 and 625 ng/L. To assess the effects of a warming scenario on the impacts of EE2, the experiments were carried out at 17 ± 1 °C (control; mean temperature of sampling area during September) and at 21 ± 1 °C (worst-case climate change scenario). The aquaria were placed in distinct climatic rooms for each temperature. To reach 21 °C, the temperature was raised by 2 °C, every 2–3 days. For each concentration level and temperature, 12 samples were considered: 4 individuals per aquarium and 3 aquaria per treatment. In each aquarium, a total of 3 L of artificial seawater (salinity: 30 ± 1), continuous aeration, and a natural photoperiod were used. The exposure medium for each condition was renewed weekly, after which EE2 concentration levels were re-established. Mortality was checked daily and found to be null. At the end of the 28-day exposure period, the clams were frozen in liquid nitrogen and stored at – 80 ºC. |
Treatment Protocol Filename: | Bivalves_Experimental_Procedure.docx |
Treatment Compound: | Sea warming and 17-α-ethinylestradiol exposure |
Treatment Dose: | Clams were exposed to five different EE2 concentrations: 0 (control group), 5, 25, 125 and 625 ng/L. To assess the effects of a warming scenario on the impacts of EE2, the experiments were carried out at 17 ± 1 °C (control) and at 21 ± 1 °C (worst-case climate change scenario). |