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MB Sample ID: SA253236
Local Sample ID: | 2243_0_TP6_2 |
Subject ID: | SU002616 |
Subject Type: | Bacteria |
Subject Species: | Eggerthella lenta |
Taxonomy ID: | 84112 |
Genotype Strain: | DSM 2243, Valencia, AB8n2 |
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Treatment:
Treatment ID: | TR002628 |
Treatment Summary: | For growth and metabolomics experiments, glycerol stocks of the 3 E. lenta strains were first streaked on BHI+ agar plates and incubated at 37°C for 2-3 days. Individual colonies were inoculated into 3-4 mL liquid BHI+ and incubated at 37°C for 40-48 hours, or until approximately early stationary phase. Culture optical density (600 nm wavelength absorbance, OD600) was measured using a Hach DR1900 spectrophotometer. 1 mL samples of BHI starter cultures were then centrifuged at 1,568 rcf for 4 minutes in a microcentrifuge (ThermoScientific mySpin 12) in the anaerobic chamber and resuspended in 1 mL sterile phosphate-buffered saline (PBS). The resulting suspension was vortexed and diluted to an approximate OD600 of 0.1, and used as inoculum into defined experimental conditions. Varying media conditions were prepared separately and all allowed to fully reduce in the anaerobic chamber prior to inoculation. |