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MB Sample ID: SA295633
Local Sample ID: | Right_Retina_2_POS |
Subject ID: | SU002882 |
Subject Type: | Fish |
Subject Species: | Danio rerio |
Taxonomy ID: | 7955 |
Gender: | Male and female |
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Treatment:
Treatment ID: | TR002891 |
Treatment Summary: | For optic nerve crush, animals were deeply anesthetized in 0.033% tricaine methane-sulfonate (MS-222). The right optic nerve was exposed by gently removing the connective tissue on the dorsal half of the eye and rotating the eye ventrally out of the orbit with a number 5 forceps. A nerve crush was then performed using number 5 forceps to crush the nerve ~0.5–1 mm from the optic nerve head for 5 s. Success was observed by the generation of a translucent stripe in the nerve that completely separated two areas of white myelination with no bleeding. Fish were then revived in fresh aquatic system water in individual tanks. After 1 h the tanks were returned to the fish system and animals were maintained normally with daily feeding until 3 days post injury. |