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MB Sample ID: SA001190
| Local Sample ID: | 95p |
| Subject ID: | SU000036 |
| Subject Type: | Animal |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000036 |
| Subject Type: | Animal |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 95p | SA001190 | FL000420 | 0.95 | %13C |
Collection:
| Collection ID: | CO000019 |
| Collection Summary: | none |
| Sample Type: | Worms |
Treatment:
| Treatment ID: | TR001190 |
| Treatment Summary: | none |
Sample Preparation:
| Sampleprep ID: | SP000032 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | IROAGlucoseNMR_Feb11_2014.txt |
| Sampleprep Protocol Comments: | 5%: 10.7 mg dissolved in 594 L of 99% D2O and 6 L of 111.11 mM d4-TSP in D2O 95%: 2.8 mg dissolved in 594 L of 99% D2O and 6 L of 111.11 mM d4-TSP in D2O Blank: 594 L of 99% D2O and 6 L of 111.11 mM d4-TSP in D2O |
Combined analysis:
| Analysis ID | AN000038 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Accela 1250 |
| Column | Thermo Scientific Gold aQ |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units |
Chromatography:
| Chromatography ID: | CH000020 |
| Chromatography Comments: | 100% solvent A for 1 min followed by a linear gradient to 20% B in 6 min, a linear gradient to 60% B in 2 min, a linear gradient to 95% B in 4 min, held for 2 min, and a 3.5 min return to the starting composition |
| Instrument Name: | Thermo Accela 1250 |
| Column Name: | Thermo Scientific Gold aQ |
| Column Temperature: | 40C |
| Flow Rate: | 600 uL/min |
| Sample Injection: | 3 uL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 18.5 min |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000035 |
| Analysis ID: | AN000038 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | LCMSmethod.rtf |
| Ion Mode: | POSITIVE |
| Mass Accuracy: | 1.5 ppm |
| Spray Voltage: | 3kV |
| Resolution Setting: | 70000 |
