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MB Sample ID: SA003961

Local Sample ID:13C-Glc-plate-2-cells
Subject ID:SU000095
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:A549
Species Group:Human

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU000095
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:A549
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
13C-Glc-plate-2-cellsSA003961FL00102113C-GlcLabeling

Collection:

Collection ID:CO000078
Collection Summary:cell_quench_collection
Collection Protocol Filename:14Jun17_Fan_Quench_cell_tissue_SOP.pdf
Collection Protocol Comments:Collection and quench of cells in tissue culture
Sample Type:Lung

Treatment:

Treatment ID:TR000096
Treatment Summary:13C-Glc|13C/15N-Gln|unlabeled
Treatment Protocol Filename:14Jun17Fan_Cell_Tracer_Ex_SOP.pdf
Treatment Protocol Comments:Cell culture in 13C-Glc-labeled media | 13C/15N-Gln-labeled media | unlabeled media for 24h

Sample Preparation:

Sampleprep ID:SP000091
Sampleprep Summary:protein_extraction|polar_preparation|polar_extraction
Sampleprep Protocol Filename:14June20Fan_Prot_Quant_SOP.pdf
14Jun30Fan_Prep_Polar_SOP.pdf
14Jun30Fan_Extract_Polar_Lipid_Prot_SOP.pdf
Sampleprep Protocol Comments:Protein extraction and quantification|Preparation of polar samples for GCMS and NMR|Polar extraction from cells.

Combined analysis:

Analysis ID AN000121 AN000122
Analysis type MS MS
Chromatography type GC GC
Chromatography system
Column
MS Type ESI EI
MS instrument type Single quadrupole GC-ITQ
MS instrument name Thermo LTQ-FT Thermo ITQ
Ion Mode NEGATIVE POSITIVE
Units normalized corrected Peak area Peak area

Chromatography:

Chromatography ID:CH000083
Chromatography Type:GC

MS:

MS ID:MS000097
Analysis ID:AN000121
Instrument Name:Thermo LTQ-FT
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:FTMS Analytical Experiment with detection of compounds with unique CHONPS molecular formula
Ion Mode:NEGATIVE
  
MS ID:MS000098
Analysis ID:AN000122
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivatization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide)
Ion Mode:POSITIVE
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