Return to study ST000287 main page

MB Sample ID: SA013128

Local Sample ID:scf_02-M5
Subject ID:SU000307
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male
Animal Animal Supplier:Jackson Lab
Animal Housing:McKnight Brain Institute, UF
Animal Light Cycle:12 hr light/12 hr dark
Animal Feed:ad libtum
Animal Water:ad libtum
Animal Inclusion Criteria:males only
Species Group:Mammal

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU000307
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male
Animal Animal Supplier:Jackson Lab
Animal Housing:McKnight Brain Institute, UF
Animal Light Cycle:12 hr light/12 hr dark
Animal Feed:ad libtum
Animal Water:ad libtum
Animal Inclusion Criteria:males only
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
scf_02-M5SA013128FL003104mdx-nNOS-/-Strain

Collection:

Collection ID:CO000301
Collection Summary:Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C
Collection Protocol Comments:L3-190
Sample Type:Muscle
Collection Method:muscle extraction, frozen
Collection Location:UF, musle physiology laboratory
Collection Frequency:once/mouse
Collection Duration:Total ~5 min
Collection Time:no dose
Volumeoramount Collected:entire muscles extracted
Storage Conditions:-80C freezer
Collection Vials:2 ml vials
Storage Vials:2 ml vials
Collection Tube Temp:-80C
Additives:none
Blood Serum Or Plasma:none
Tissue Cell Identification:none
Tissue Cell Quantity Taken:none

Treatment:

Treatment ID:TR000321
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP000315
Sampleprep Summary:NAD/ATP or NADH
Sampleprep Protocol Filename:SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf

Combined analysis:

Analysis ID AN000456 AN000457
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Thermo Hypercarb (50 x 2.1mm, 3um) Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Agilent 6490 QQQ
Ion Mode POSITIVE POSITIVE
Units pmol/mg dry powder pmol/mg dry powder

Chromatography:

Chromatography ID:CH000325
Chromatography Summary:NAD-ATP method
Methods Filename:SB_NAD-ATP_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Thermo Hypercarb (50 x 2.1mm, 3um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000326
Chromatography Summary:NAD-ATP method
Methods Filename:SB_NADH_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000396
Analysis ID:AN000456
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NAD/ATP
Ion Mode:POSITIVE
Analysis Protocol File:SB_NAD-ATP_Assay_Agilent.pdf
  
MS ID:MS000397
Analysis ID:AN000457
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NADH
Ion Mode:POSITIVE
Analysis Protocol File:SB_NADH_Assay_Agilent.pdf
  logo