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MB Sample ID: SA015177
| Local Sample ID: | DNA-20 |
| Subject ID: | SU000361 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6 |
| Age Or Age Range: | 15 weeks |
| Weight Or Weight Range: | 20-30g |
| Gender: | Female |
| Animal Water: | with or without Zn |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000361 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6 |
| Age Or Age Range: | 15 weeks |
| Weight Or Weight Range: | 20-30g |
| Gender: | Female |
| Animal Water: | with or without Zn |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| DNA-20 | SA015177 | FL003376 | Bone Marrow | Tissue |
| DNA-20 | SA015177 | FL003376 | Zn- | Treatment |
Collection:
| Collection ID: | CO000355 |
| Collection Summary: | DNA will be provided in TE solution. Concentrations are on Metadata sheet. |
| Collection Protocol Filename: | MeDNA_SOP.pdf |
| Sample Type: | Liver DNA in 10mM Tris-HCl, 1 mM EDTA(pH 7.5) & Bone Marrow DNA in 10mM Tris-HCl, 0.5mM EDTA (pH 9.0) |
| Collection Method: | Dissection followed by DNA prep |
| Collection Location: | Our Lab |
| Collection Frequency: | once |
| Collection Duration: | A few minutes. |
| Volumeoramount Collected: | 19-20 ul |
| Storage Conditions: | -20C |
| Storage Vials: | eppendorf tubes |
Treatment:
| Treatment ID: | TR000375 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP000368 |
| Sampleprep Summary: | See file attached. |
| Sampleprep Protocol Filename: | MeDNA_SOP.pdf |
Combined analysis:
| Analysis ID | AN000553 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Accela |
| Column | Phenomenex Luna C18 (150 x 2mm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantum Ultra |
| Ion Mode | POSITIVE |
| Units | µmol/l |
Chromatography:
| Chromatography ID: | CH000390 |
| Chromatography Summary: | See file |
| Methods Filename: | MeDNA_SOP.pdf |
| Instrument Name: | Thermo Accela |
| Column Name: | Phenomenex Luna C18 (150 x 2mm) |
| Flow Rate: | 300 uL/min. |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000489 |
| Analysis ID: | AN000553 |
| Instrument Name: | Thermo Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
