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MB Sample ID: SA025810
| Local Sample ID: | iPSC_09 |
| Subject ID: | SU000520 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000520 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| iPSC_09 | SA025810 | FL006028 | KO/KO | Genotype |
Collection:
| Collection ID: | CO000514 |
| Collection Summary: | Cells were washed once with cold PBS, scraped off the plate in 1 ml cold PBS, transfered in pre-cooled 50 ml falcons and pelleted by centrifugation 3000rpm at 4°C. PBS was taken off and pellets were then snap frozen in liquid N2 and stored at -80°C. |
| Sample Type: | Neurons |
Treatment:
| Treatment ID: | TR000534 |
| Treatment Summary: | 80% confluence, 48 hrs after last media change, differentiation medium (Neurobasal/B27/L-glut containing medium supplemented with BDNF, ascorbic acid , GDNF, TGFβ3, dibutyryl cAMP and DAPT. |
Sample Preparation:
| Sampleprep ID: | SP000527 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Combined analysis:
| Analysis ID | AN000767 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Thermo Hypercarb (50 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | nmol/mg protein |
Chromatography:
| Chromatography ID: | CH000552 |
| Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000679 |
| Analysis ID: | AN000767 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
