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MB Sample ID: SA057100

Local Sample ID:20150505_NK_plate1-sup
Subject ID:SU000988
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU000988
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
20150505_NK_plate1-supSA057100FL010280NKcell_type

Collection:

Collection ID:CO000982
Collection Summary:Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate.
Collection Protocol ID:blood_collection cell_quench_collection cells_media
Collection Protocol Filename:WS_Fan_Quench_cell_tissue.pdf
Sample Type:T-Cells

Treatment:

Treatment ID:TR001002
Treatment Summary:Grown in unlabeled glucose. Grown in labeled glucose. Normal human, no treatment.
Treatment Protocol ID:12CGlc 13CGlc normal

Sample Preparation:

Sampleprep ID:SP000995
Sampleprep Summary:Cells of interest extracted from the blood. Polar extraction from cells.
Sampleprep Protocol ID:cell_extraction polar_extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf

Combined analysis:

Analysis ID AN001558
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units normalized peak area

Chromatography:

Chromatography ID:CH001092
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

MS:

MS ID:MS001436
Analysis ID:AN001558
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).
Ion Mode:POSITIVE
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