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MB Sample ID: SA057107

Local Sample ID:20150602_T_memory_plate12-cells
Subject ID:SU000989
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU000989
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
20150602_T_memory_plate12-cellsSA057107FL010281CD8_T_memorycell_type

Collection:

Collection ID:CO000983
Collection Summary:Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate.
Collection Protocol ID:blood_collection cell_quench_collection cells_media
Collection Protocol Filename:WS_Fan_Quench_cell_tissue.pdf
Sample Type:T-Cells

Treatment:

Treatment ID:TR001003
Treatment Summary:Grown in unlabeled glucose and treated with galactose. Grown in labeled glucose and treated with galactose. Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose.
Treatment Protocol ID:galactose_12CGlc galactose_13CGlc normal normal_12CGlc normal_13CGlc

Sample Preparation:

Sampleprep ID:SP000996
Sampleprep Summary:Cells of interest extracted from the blood. Polar extraction from cells.
Sampleprep Protocol ID:cell_extraction polar_extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf

Combined analysis:

Analysis ID AN001559
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units normalized peak area

Chromatography:

Chromatography ID:CH001093
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

MS:

MS ID:MS001437
Analysis ID:AN001559
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).
Ion Mode:POSITIVE
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