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MB Sample ID: SA057121
| Local Sample ID: | 20150818_T_naive_plate6 |
| Subject ID: | SU000990 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000990 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 20150818_T_naive_plate6 | SA057121 | FL010285 | CD8_T_naive | cell_type |
Collection:
| Collection ID: | CO000984 |
| Collection Summary: | Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate. |
| Collection Protocol ID: | blood_collection cell_quench_collection cells_media |
| Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
| Sample Type: | T-Cells |
Treatment:
| Treatment ID: | TR001004 |
| Treatment Summary: | Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose. Grown in unlabeled glucose and treated with oligomycin. Grown in labeled glucose and treated with oligomycin. |
| Treatment Protocol ID: | normal normal_12CGlc normal_13CGlc oligomycin_12CGlc oligomycin_13CGlc |
Sample Preparation:
| Sampleprep ID: | SP000997 |
| Sampleprep Summary: | Cells of interest extracted from the blood. Polar extraction from cells. |
| Sampleprep Protocol ID: | cell_extraction polar_extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf |
Combined analysis:
| Analysis ID | AN001560 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | GC-EI-MS, Thermo ITQ |
| Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| MS Type | EI |
| MS instrument type | GC-ITQ |
| MS instrument name | Thermo ITQ |
| Ion Mode | POSITIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH001094 |
| Chromatography Summary: | Targeted GC |
| Instrument Name: | GC-EI-MS, Thermo ITQ |
| Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001438 |
| Analysis ID: | AN001560 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Ion Mode: | POSITIVE |
