Return to study ST000952 main page
MB Sample ID: SA057134
Local Sample ID: | 20150821_NK_plate5 |
Subject ID: | SU000991 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000991 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
20150821_NK_plate5 | SA057134 | FL010288 | NK | cell_type |
Collection:
Collection ID: | CO000985 |
Collection Summary: | Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate. |
Collection Protocol ID: | blood_collection cell_quench_collection cells_media |
Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
Sample Type: | T-Cells |
Treatment:
Treatment ID: | TR001005 |
Treatment Summary: | Grown in unlabeled glucose and treated with galactose. Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose. Grown in unlabeled glucose and treated with oligomycin. Grown in labeled glucose and treated with oligomycin. |
Treatment Protocol ID: | galactose_12CGlc normal normal_12CGlc normal_13CGlc oligomycin_12CGlc oligomycin_13CGlc |
Sample Preparation:
Sampleprep ID: | SP000998 |
Sampleprep Summary: | Cells of interest extracted from the blood. Polar extraction from cells. |
Sampleprep Protocol ID: | cell_extraction polar_extraction |
Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf |
Combined analysis:
Analysis ID | AN001561 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | GC-EI-MS, Thermo ITQ |
Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
MS Type | EI |
MS instrument type | GC-ITQ |
MS instrument name | Thermo ITQ |
Ion Mode | POSITIVE |
Units | normalized peak area |
Chromatography:
Chromatography ID: | CH001095 |
Chromatography Summary: | Targeted GC |
Instrument Name: | GC-EI-MS, Thermo ITQ |
Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
Chromatography Type: | GC |
MS:
MS ID: | MS001439 |
Analysis ID: | AN001561 |
Instrument Name: | Thermo ITQ |
Instrument Type: | GC-ITQ |
MS Type: | EI |
MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
Ion Mode: | POSITIVE |