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MB Sample ID: SA057141

Local Sample ID:EW-06-RCH
Subject ID:SU000992
Subject Type:Insect
Subject Species:Aphaenogaster picea
Taxonomy ID:602698
Genotype Strain:Field colonies of forest ant

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Subject:

Subject ID:SU000992
Subject Type:Insect
Subject Species:Aphaenogaster picea
Taxonomy ID:602698
Genotype Strain:Field colonies of forest ant

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
EW-06-RCHSA057141FL010290RCHTreatment

Collection:

Collection ID:CO000986
Collection Summary:Live animals flash-frozen in liquid nitrogen and kept at -80. Sample source is whole animal tissue (~40 workers/sample)
Sample Type:Whole Tissue

Treatment:

Treatment ID:TR001006
Treatment Summary:Control - at 25 degrees C. Cold shock - 0 degrees C for one hour, recovery at 25 degrees one hour. Acclimation - two weeks at 4 degrees C. Acronyms used in study design for treatment (ACC - acclimation, RCH - cold shock, RT - room temperature)

Sample Preparation:

Sampleprep ID:SP000999
Sampleprep Summary:none
Sampleprep Protocol Filename:GMetabolomics_LCMS_Protocol_092117.pdf
Appendix_A_Internal_Standard_Prep_GLCMS.pdf
Sample Spiking:Appendix_A_Internal_Standard_Prep_GLCMS.pdf

Combined analysis:

Analysis ID AN001562 AN001563
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Height peak height

Chromatography:

Chromatography ID:CH001096
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:350 ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001440
Analysis ID:AN001562
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001441
Analysis ID:AN001563
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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