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MB Sample ID: SA057143
| Local Sample ID: | EW-10-RCH |
| Subject ID: | SU000992 |
| Subject Type: | Insect |
| Subject Species: | Aphaenogaster picea |
| Taxonomy ID: | 602698 |
| Genotype Strain: | Field colonies of forest ant |
| Species Group: | Insects |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000992 |
| Subject Type: | Insect |
| Subject Species: | Aphaenogaster picea |
| Taxonomy ID: | 602698 |
| Genotype Strain: | Field colonies of forest ant |
| Species Group: | Insects |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| EW-10-RCH | SA057143 | FL010290 | RCH | Treatment |
Collection:
| Collection ID: | CO000986 |
| Collection Summary: | Live animals flash-frozen in liquid nitrogen and kept at -80. Sample source is whole animal tissue (~40 workers/sample) |
| Sample Type: | Whole Tissue |
Treatment:
| Treatment ID: | TR001006 |
| Treatment Summary: | Control - at 25 degrees C. Cold shock - 0 degrees C for one hour, recovery at 25 degrees one hour. Acclimation - two weeks at 4 degrees C. Acronyms used in study design for treatment (ACC - acclimation, RCH - cold shock, RT - room temperature) |
Sample Preparation:
| Sampleprep ID: | SP000999 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | GMetabolomics_LCMS_Protocol_092117.pdf Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
| Sample Spiking: | Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
Combined analysis:
| Analysis ID | AN001562 | AN001563 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Height | peak height |
Chromatography:
| Chromatography ID: | CH001096 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350 ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001440 |
| Analysis ID: | AN001562 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001441 |
| Analysis ID: | AN001563 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
