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MB Sample ID: SA057294

Local Sample ID:BLS002A
Subject ID:SU000996
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU000996
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
BLS002ASA057294FL010320fatMilk fraction

Collection:

Collection ID:CO000990
Collection Summary:mothers will arrive in a fasted state in the morning and complete an infant feeding questionnaire as described previously (Lemas et al. 2016). Mothers will then pump ~30mL human milk (HM) into polypropylene vials and stored at -80ÂșC.
Sample Type:Human Milk

Treatment:

Treatment ID:TR001010
Treatment Summary:human milk samples were fractionated (fat and skim) using centrifugation and whole milk samples wer snap frozen at -80c.

Sample Preparation:

Sampleprep ID:SP001003
Sampleprep Summary:None
Sampleprep Protocol Filename:GMetabolomics_LCMS_Protocol_092117.pdf
Appendix_A_Internal_Standard_Prep_GLCMS.pdf

Combined analysis:

Analysis ID AN001570 AN001571
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak height peak height

Chromatography:

Chromatography ID:CH001100
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:350 ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001448
Analysis ID:AN001570
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001449
Analysis ID:AN001571
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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