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MB Sample ID: SA070424

Local Sample ID:5_ FASNsh_ no label Glc
Subject ID:SU001086
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU001086
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
5_ FASNsh_ no label GlcSA070424FL01117812CGlc_FASN_shRNAprotocol.id

Collection:

Collection ID:CO001080
Collection Summary:Collection and quench of cells in tissue culture Media is extracted from plate.
Collection Protocol ID:cell_quench_collection cells_media
Collection Protocol Filename:WS_Fan_Quench_cell_tissue.pdf
Sample Type:Cultured cells

Treatment:

Treatment ID:TR001100
Treatment Summary:Grown in unlabeled glucose and transfected with FASN shRNA. Grown in unlabeled glucose and transfected with non targeted shRNA. Grown in labeled glucose and transfected with FASN shRNA. Grown in labeled glucose and transfected with non targeted shRNA.
Treatment Protocol ID:12CGlc_FASN_shRNA 12CGlc_NTC_shRNA 13CGlc_FASN_shRNA 13CGlc_NTC_shRNA

Sample Preparation:

Sampleprep ID:SP001093
Sampleprep Summary:Lipid extraction from cells. Polar extraction from cells. Protein extraction and quantification.
Sampleprep Protocol ID:lipid_extraction polar_extraction protein_extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf
WS_Fan_Prot_Quant.pdf

Combined analysis:

Analysis ID AN001710
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units normalized peak area

Chromatography:

Chromatography ID:CH001207
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

MS:

MS ID:MS001583
Analysis ID:AN001710
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).
Ion Mode:POSITIVE
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