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MB Sample ID: SA078274
Local Sample ID: | 7_MDA231_13C15NGln_shP4HA1_rep2-cells-polar_GCMS_A |
Subject ID: | SU001199 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001199 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
7_MDA231_13C15NGln_shP4HA1_rep2-cells-polar_GCMS_A | SA078274 | FL011930 | 13C15NGln_shP4HA1 | protocol.id |
Collection:
Collection ID: | CO001193 |
Collection Summary: | Collection and quench of cells in culture |
Collection Protocol ID: | cell_quench_collection |
Collection Protocol Filename: | 3A_Cells_Quench_Cell_Fan_20170412.pdf |
Sample Type: | cultured cells |
Treatment:
Treatment ID: | TR001214 |
Treatment Summary: | Cells grown in unlabeled glutamine and infected with empty vector. Cells grown in unlabeled glutamine and infected with shP4HA1 lentivirus. Cells grown in labeled glutamine and infected with empty vector. Cells grown in labeled glutamine and infected with shP4HA1 lentivirus. |
Treatment Protocol ID: | 12C14NGln_shCTL 12C14NGln_shP4HA1 13C15NGln_shCTL 13C15NGln_shP4HA1 |
Sample Preparation:
Sampleprep ID: | SP001207 |
Sampleprep Summary: | Polar extraction from cells. Protein extraction and quantification. |
Sampleprep Protocol ID: | polar_extraction protein_extraction |
Sampleprep Protocol Filename: | 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf 4D_17Jun4_Fan_Prot_Quant.pdf |
Combined analysis:
Analysis ID | AN001865 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | GC-EI-MS, Thermo ITQ |
Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
MS Type | EI |
MS instrument type | GC-ITQ |
MS instrument name | Thermo ITQ |
Ion Mode | POSITIVE |
Units | normalized peak area |
Chromatography:
Chromatography ID: | CH001350 |
Chromatography Summary: | Targeted GC |
Instrument Name: | GC-EI-MS, Thermo ITQ |
Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
Chromatography Type: | GC |
MS:
MS ID: | MS001725 |
Analysis ID: | AN001865 |
Instrument Name: | Thermo ITQ |
Instrument Type: | GC-ITQ |
MS Type: | EI |
MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
Ion Mode: | POSITIVE |