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MB Sample ID: SA078274

Local Sample ID:7_MDA231_13C15NGln_shP4HA1_rep2-cells-polar_GCMS_A
Subject ID:SU001199
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU001199
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
7_MDA231_13C15NGln_shP4HA1_rep2-cells-polar_GCMS_ASA078274FL01193013C15NGln_shP4HA1protocol.id

Collection:

Collection ID:CO001193
Collection Summary:Collection and quench of cells in culture
Collection Protocol ID:cell_quench_collection
Collection Protocol Filename:3A_Cells_Quench_Cell_Fan_20170412.pdf
Sample Type:cultured cells

Treatment:

Treatment ID:TR001214
Treatment Summary:Cells grown in unlabeled glutamine and infected with empty vector. Cells grown in unlabeled glutamine and infected with shP4HA1 lentivirus. Cells grown in labeled glutamine and infected with empty vector. Cells grown in labeled glutamine and infected with shP4HA1 lentivirus.
Treatment Protocol ID:12C14NGln_shCTL 12C14NGln_shP4HA1 13C15NGln_shCTL 13C15NGln_shP4HA1

Sample Preparation:

Sampleprep ID:SP001207
Sampleprep Summary:Polar extraction from cells. Protein extraction and quantification.
Sampleprep Protocol ID:polar_extraction protein_extraction
Sampleprep Protocol Filename:4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf
4D_17Jun4_Fan_Prot_Quant.pdf

Combined analysis:

Analysis ID AN001865
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Ion Mode POSITIVE
Units normalized peak area

Chromatography:

Chromatography ID:CH001350
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

MS:

MS ID:MS001725
Analysis ID:AN001865
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).
Ion Mode:POSITIVE
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