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MB Sample ID: SA123795

Local Sample ID:11_B1-1_Colon_syngenic_7days_170427_UKy_GCH_rep2-lipid
Subject ID:SU001523
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU001523
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
11_B1-1_Colon_syngenic_7days_170427_UKy_GCH_rep2-lipidSA123795FL014994syngenicTreatment Protocol

Collection:

Collection ID:CO001518
Collection Summary:Mouse is sacrificed and tissues are harvested.
Collection Protocol ID:mouse_tissue_collection
Sample Type:Multiple tissues

Treatment:

Treatment ID:TR001538
Treatment Summary:Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest.
Treatment Protocol ID:allogenic naive syngenic

Sample Preparation:

Sampleprep ID:SP001531
Sampleprep Summary:Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. Lipid extraction from homogenate. Protein extraction and quantification. Tissue is frozen in liquid nitrogen to stop metabolic processes.
Sampleprep Protocol ID:frozen_tissue_grind lipid_extraction protein_extraction tissue_quench
Sampleprep Protocol Filename:4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf
4D_17Jun4_Fan_Prot_Quant.pdf
4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf

Combined analysis:

Analysis ID AN002420
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system None
Column None
MS Type ESI
MS instrument type FTMS
MS instrument name Orbitrap Fusion
Ion Mode POSITIVE
Units peak_height

Chromatography:

Chromatography ID:CH001777
Chromatography Summary:None
Instrument Name:None
Column Name:None
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS002257
Analysis ID:AN002420
Instrument Name:Orbitrap Fusion
Instrument Type:FTMS
MS Type:ESI
MS Comments:Measurements made using direct infusion (nano-electrospray) FTMS.
Ion Mode:POSITIVE
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