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MB Sample ID: SA268827

Local Sample ID:Fig3 N6 20min 13C Gluc
Subject ID:SU002803
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Subject:

Subject ID:SU002803
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Fig3 N6 20min 13C GlucSA268827FL03516410min 13C GlucTreatment

Collection:

Collection ID:CO002796
Collection Summary:Hippocampal slices were stimulated with 50mM potassium chloride over a time course of 5 minutes. The experiment was terminated with thermal preservation at the indicated time points.
Sample Type:Brain

Treatment:

Treatment ID:TR002812
Treatment Summary:50mM potassium chloride

Sample Preparation:

Sampleprep ID:SP002809
Sampleprep Summary:At the specific time chosen for thermal preservation, the slices were flash heated to 80°C for 2 seconds and then cooled to 4°C; the slices were then flash-frozen to -20 °C. The brain slices were stored at -80°C for subsequent thin sectioning in a cryomicrotome. The thin sections were mounted onto conductive glass slides (ITO slides) and sprayed with matrix (1,5-diaminonaphthalene).

Combined analysis:

Analysis ID AN004374
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type MALDI
MS instrument type QTOF
MS instrument name Bruker timsTOF fleX
Ion Mode NEGATIVE
Units a.u.

Chromatography:

Chromatography ID:CH003280
Instrument Name:none
Column Name:none
Column Temperature:none
Flow Gradient:none
Flow Rate:none
Solvent A:none
Solvent B:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS004123
Analysis ID:AN004374
Instrument Name:Bruker timsTOF fleX
Instrument Type:QTOF
MS Type:MALDI
MS Comments:Mass spectra were acquired on a timsTOF fleX in negative ion mode. Hippocampal sections were outlined as individual ROIs with a pixel size of 20 µm. Most metabolites were acquired in a single imaging run (m/z range 50 - 1000). Each MSI pixel consisted of 1000 laser shots. The laser Smartbeam parameter was set to single with a frequency of 10 kHz.
Ion Mode:NEGATIVE
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