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MB Sample ID: SA318111

Local Sample ID:WT C2C12 UnT5 M_Dasarathy_Nicole_114Cells_02042022_HILIC_POS202
Subject ID:SU003043
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:Passage 4-8
Gender:Female
Species Group:Mammals

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Subject:

Subject ID:SU003043
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:Passage 4-8
Gender:Female
Species Group:Mammals

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
WT C2C12 UnT5 M_Dasarathy_Nicole_114Cells_02042022_HILIC_POS202SA318111FL037860Wild-typeGenotype
WT C2C12 UnT5 M_Dasarathy_Nicole_114Cells_02042022_HILIC_POS202SA318111FL037860UntreatedTreatment

Collection:

Collection ID:CO003036
Collection Summary:Metabolites were extracted from myotubes and skeletal muscle using chilled methanol containing 6 labeled internal standards (Betaine-d9, Carnitine-d9, Estrone-13C3, Cholesterol-13C3, Valeric acid-d9, Choline-d13). After centrifugation at 14,000g for 20 minutes to precipitate the protein pellet, the supernatant was removed, dried, and reconstituted in 5% acetonitrile. LC/MS (liquid chromatography/mass spectrometry) analysis was then performed, including on pooled quality control samples.
Sample Type:Skeletal myotubes

Treatment:

Treatment ID:TR003052
Treatment Summary:Cells were not treated (Untreated, UnT)

Sample Preparation:

Sampleprep ID:SP003049
Sampleprep Summary:Metabolites were extracted from myotubes and skeletal muscle using chilled methanol containing 6 labeled internal standards (Betaine-d9, Carnitine-d9, Estrone-13C3, Cholesterol-13C3, Valeric acid-d9, Choline-d13). After centrifugation at 14,000g for 20 minutes to precipitate the protein pellet, the supernatant was removed, dried, and reconstituted in 5% acetonitrile. LC/MS (liquid chromatography/mass spectrometry) analysis was then performed, including on pooled quality control samples.

Combined analysis:

Analysis ID AN004807 AN004808
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTRAP QTRAP
MS instrument name Thermo Q Exactive Focus Thermo Q Exactive Focus
Ion Mode POSITIVE NEGATIVE
Units relative abundance relative abundance

Chromatography:

Chromatography ID:CH003633
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:Time (min.) Flow Rate (mL/min) %B 0.0 0.2 100 2.0 0.2 70 4.0 0.2 60 5.5 0.2 60 10.5 0.2 50 11.5 0.2 50 12.0 0.2 100 16.0 0.2 100
Flow Rate:0.2 mL/min
Solvent A:100% water;10 mM ammonium acetate; 0.125% acetic acid
Solvent B:5% water/95% acetonitrile;10 mM ammonium acetate; 0.125% acetic acid
Chromatography Type:HILIC

MS:

MS ID:MS004553
Analysis ID:AN004807
Instrument Name:Thermo Q Exactive Focus
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0.
Ion Mode:POSITIVE
  
MS ID:MS004554
Analysis ID:AN004808
Instrument Name:Thermo Q Exactive Focus
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0.
Ion Mode:NEGATIVE
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