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MB Sample ID: SA005402
| Local Sample ID: | T30-2 |
| Subject ID: | SU000114 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 9 months |
| Gender: | Female |
| Animal Housing: | Singly housed |
| Animal Feed: | High fat diet (HFD, 4.73 kcal/g, 20% protein, 35% carbohydrate, and 45% fat per kcal; D12451, Research Diets, New Brunswick, NJ) |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000151 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UPLC |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex API 6500 QTrap |
| Ion Mode | NEGATIVE |
| Units | % abundance |
Chromatography:
| Chromatography ID: | CH000107 |
| Chromatography Summary: | Targeted UPLC-MS/MS method |
| Methods Filename: | Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 60 C |
| Flow Gradient: | Yes |
| Flow Rate: | 0.4mL/min |
| Internal Standard: | See protocol/methods file |
| Sample Injection: | 5 L |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 16 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 L |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 L |
| Sample Loop Size: | 17 L |
| Chromatography Type: | Reversed phase |
