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MB Sample ID: SA071800
| Local Sample ID: | 8376 |
| Subject ID: | SU001104 |
| Subject Type: | Fish |
| Subject Species: | Oreochromis mossambicus |
| Taxonomy ID: | 8127 |
| Age Or Age Range: | 1 to 14 years |
| Weight Or Weight Range: | 0.1 to 5.6 kg |
| Height Or Height Range: | NA |
| Gender: | Male and female |
| Species Group: | Fish |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001733 |
|---|---|
| Chromatography ID | CH001225 |
| MS ID | MS001602 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Fusion Tribrid Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | mg/kg (relative quantification, NOT ABSOLUTE) |
Chromatography:
| Chromatography ID: | CH001225 |
| Chromatography Summary: | A Thermo Scientific Vanquish UHPLC system (Thermo Scientific, San Jose, CA) was coupled to the Orbitrap Fusion Lumos Tribrid for the chromatographic separation of lipids. The autosampler temperature was maintained at 4 �C for all experiments. Solvent extraction blanks and quality control samples were jointly analyzed over the course of a batch (10�15 samples). A Waters Acquity C18 BEH column (2.1 � 100 mm, 1.7 ?m particle size, Waters, Milford, MA) maintained at 60 �C was used for all lipidomic studies. The mobile phase flow rate was 450 ?L/min. The gradient program consisted of mobile phase C [60:40 acetonitrile/water] and mobile phase D [90:8:2 isopropanol/ acetonitrile/water], each containing 10 mM ammonium formate and 0.1% formic acid. The gradient included 32% D at 0 min, 40% D at 1 min, a hold at 40% D until 1.5 min, 45% D at 4 min, 50% D at 5 min, 60% D at 8 min, 70% D at 11 min, 80% D at 14 min, 100% D at 16 min, and a hold at 100% D until 17 min. The total run time was 22 min, which included a 5-min equilibration. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 60 C |
| Flow Gradient: | The gradient included 32% D at 0 min, 40% D at 1 min, a hold at 40% D until 1.5 min, 45% D at 4 min, 50% D at 5 min, 60% D at 8 min, 70% D at 11 min, 80% D at 14 min, 100% D at 16 min, and a hold at 100% D until 17 min. The total run time was 22 min, which included a 5-min equilibration. |
| Flow Rate: | 450 uL/min |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
