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MB Sample ID: SA255609

Local Sample ID:h1
Subject ID:SU002643
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:MDA-MB-231

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Combined analysis:

Analysis ID AN004190
Analysis type MS
Chromatography type GC
Chromatography system HP GCD 1800B
Column Agilent PoraBOND Q (25m x 0.32mm x 0.5um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent/HP 5972 MSD
Units pg/hr/ug and g/hr for media


Chromatography ID:CH003104
Chromatography Summary:Collected canister samples were transferred to a liquid nitrogen trap through pressure differential. Pressure change between beginning and end of “injection” was measured, allowing calculation of the moles of canister collected air injected. Sample in the trap was then transferred, via heated helium flow, to an Aglient/HP 5972 MSD system (Santa Clara, CA, USA) equipped with a PoraBond Q column (25 m x 0.32 mm x 0.5 μm film thickness) (Restek©, Bellefonte, PN, USA). Targeted samples were analyzed in selected ion monitoring (SIM) mode, and untargeted samples in full scan (SCAN) mode with the mass range of 45-200 amu. The mass spectrometer was operated in electron impact ionization mode with 70 eV ionization energy, and transfer line, ion source, and quadrupole temperatures of 250, 280 and 280, respectively. For details on SIM and significantly altered, identified SCAN compounds, see Table 1. All samples were analysed within 6 days of collection. The oven program for both SIM and SCAN analyses were identical and are as follows: 35 ˚C for 2 min, 10 ˚C/min to 155 ˚C, 1 ˚C/min to 131 ˚C and 25 ˚C/min to 250 with a 5 min 30 second hold.
Instrument Name:HP GCD 1800B
Column Name:Agilent PoraBOND Q (25m x 0.32mm x 0.5um)
Column Temperature:250
Flow Gradient:NA
Flow Rate:10ml/min
Solvent A:NA
Solvent B:NA
Chromatography Type:GC