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MB Sample ID: SA318103
| Local Sample ID: | HIF KO C2C12 UnT5 M_Dasarathy_Nicole_114Cells_02042022_HILIC_POS253 |
| Subject ID: | SU003043 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | Passage 4-8 |
| Gender: | Female |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004807 | AN004808 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | Thermo Q Exactive Focus | Thermo Q Exactive Focus |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | relative abundance | relative abundance |
Chromatography:
| Chromatography ID: | CH003633 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | Time (min.) Flow Rate (mL/min) %B 0.0 0.2 100 2.0 0.2 70 4.0 0.2 60 5.5 0.2 60 10.5 0.2 50 11.5 0.2 50 12.0 0.2 100 16.0 0.2 100 |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 100% water;10 mM ammonium acetate; 0.125% acetic acid |
| Solvent B: | 5% water/95% acetonitrile;10 mM ammonium acetate; 0.125% acetic acid |
| Chromatography Type: | HILIC |
