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MB Sample ID: SA353327
| Local Sample ID: | Serum_FF_219 |
| Subject ID: | SU003357 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005303 | AN005304 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Brownlee SPP C18 (75 x 2.1mm, 2.7um) | Brownlee SPP C18 (75 x 2.1mm, 2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized to the median value of total lipid signals | Normalized to the median value of total lipid signals |
Chromatography:
| Chromatography ID: | CH004012 |
| Chromatography Summary: | Brownlee SPP C18 column (2.1 mm × 75 mm, 2.7 μm particle size); Solvent A is 100% H2O containing 0.1% formic acid and 1% of 1 M NH4OAc; Solvent B is 1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH4OAc |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Brownlee SPP C18 (75 x 2.1mm, 2.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0–2 min 35% B, 2–8 min from 35 to 80% B, 8–22 min from 80 to 99% B, 22–36 min 99% B, and 36.1–40 min from 99 to 35% B. |
| Flow Rate: | 0.400 mL/min |
| Solvent A: | 100% water; 0.1% formic acid; 10mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol; 0.1% formic acid; 10mM ammonium acetate |
| Chromatography Type: | Reversed phase |
