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MB Sample ID: SA028283
| Local Sample ID: | CS44_MUT04_48 |
| Subject ID: | SU000570 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
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Collection:
| Collection ID: | CO000564 |
| Collection Summary: | 293T Cells were harvested 24 and 48 hours after transfection by removal of media and rapid quenching with 1.5 mL per 10 cm plate of -80°C methanol. Cells were incubated at -80°C for 15 minutesmin, then scraped off the dish and centrifuged for 5 min at 2,000 x g at 4°C to pellet cellular debris. Pellet was re-extracted by addition of 500 µL of -80°C 80% methanol in water, vortexed, then incubated at 4°C for 15 minutes and centrifuged for 5 minutes at 2,000 x g at 4°C. Peripheral blood mono-nuclear cells were centrifuged at 2,000 x g, freeze media removed and extracted using same procedure as 293T cells. |
| Collection Protocol Filename: | The common feature of IDH1 and IDH2 mutations.pdf |
| Sample Type: | Cells |
| Collection Location: | UC Davis Genome and Biomedical Sciences Facility |
