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MB Sample ID: SA091167
| Local Sample ID: | Patrick Media 3 |
| Subject ID: | SU001320 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO001314 |
| Collection Summary: | pDFs obtained from WT, KI, and KO mice were plated at a density of 2 x 106 on 10 cm tissue culture plates in high glucose DMEM supplemented with 20% FBS (Gibco) and 2% penicillin/streptomycin. Following the overnight incubation cells were rested in 2% FBS (Gibco) 2% penicillin/streptomycin (Bio Whittaker) high glucose DMEM (Gibco) for 2 hours, then the media was exchanged with 0% FBS 2% penicillin/ high glucose DMEM, and mechanical trauma was induced on the monolayer by performing scratched across the diameter of the plate in an asterisk pattern using 4 x 20 ul pipette tips on a multichannel micro pipettor. Media was taken for lipidomic analysis at 0 h and 2 h. |
| Sample Type: | Fibroblasts |
