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MB Sample ID: SA218361

Local Sample ID:1024025750
Subject ID:SU002364
Subject Type:Fish
Subject Species:Oncorhynchus mykiss

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Collection ID:CO002357
Collection Summary:For the metabolite and microbiota analyses, 10 fish from the control group were collected on day 0 and 10 fish from each group (control, 0.1, 1.0, 5.0% β-glucan) at week 6. Fish were euthanized by immersion in an overdose (200 mg∙L-1) of MS-222 (cat no. A5040, Sigma-Aldrich). The tail was cut, and blood was sampled from the vena caudalis using Na-heparinised 25 mL and 50 mL capillary pipettes (Hirschmann Laborgeräte, Germany). Blood samples were centrifuged at 3,000 g for 10 min at 4 °C, and serum was isolated and stored at -80 °C. The intestine was aseptically sampled and immediately immersed in RNAlater® (Sigma-Aldrich) and transferred to -20 °C.
Sample Type:Blood (serum)
Storage Conditions:-20℃