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MB Sample ID: SA318103
| Local Sample ID: | HIF KO C2C12 UnT5 M_Dasarathy_Nicole_114Cells_02042022_HILIC_POS253 |
| Subject ID: | SU003043 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | Passage 4-8 |
| Gender: | Female |
| Species Group: | Mammals |
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Collection:
| Collection ID: | CO003036 |
| Collection Summary: | Metabolites were extracted from myotubes and skeletal muscle using chilled methanol containing 6 labeled internal standards (Betaine-d9, Carnitine-d9, Estrone-13C3, Cholesterol-13C3, Valeric acid-d9, Choline-d13). After centrifugation at 14,000g for 20 minutes to precipitate the protein pellet, the supernatant was removed, dried, and reconstituted in 5% acetonitrile. LC/MS (liquid chromatography/mass spectrometry) analysis was then performed, including on pooled quality control samples. |
| Sample Type: | Skeletal myotubes |
