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MB Sample ID: SA318111

Local Sample ID:WT C2C12 UnT5 M_Dasarathy_Nicole_114Cells_02042022_HILIC_POS202
Subject ID:SU003043
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:Passage 4-8
Gender:Female
Species Group:Mammals

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Combined analysis:

Analysis ID AN004807 AN004808
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTRAP QTRAP
MS instrument name Thermo Q Exactive Focus Thermo Q Exactive Focus
Ion Mode POSITIVE NEGATIVE
Units relative abundance relative abundance

MS:

MS ID:MS004553
Analysis ID:AN004807
Instrument Name:Thermo Q Exactive Focus
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0.
Ion Mode:POSITIVE
  
MS ID:MS004554
Analysis ID:AN004808
Instrument Name:Thermo Q Exactive Focus
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Data dependent acquisitions (DDA) on the pooled representative QC samples include MS full scans at a resolution of 120,000 and HCD MS/MS scans taken on the top 5 most abundant ions at a resolution of 30,000 with dynamic exclusion. The resolution of the MS2 scans were taken at a stepped NCE energy of 10.0, 20.0 and 30.0.
Ion Mode:NEGATIVE
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