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MB Sample ID: SA320903
| Local Sample ID: | WT1 |
| Subject ID: | SU003060 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004832 | AN004833 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap Exploris 120 | Thermo Orbitrap Exploris 120 |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
MS:
| MS ID: | MS004578 |
| Analysis ID: | AN004832 |
| Instrument Name: | Thermo Orbitrap Exploris 120 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The mass spectrometry analysis was processed using an Orbitrap Exploris 120 mass spectrometer Thermo Fisher (Waltham, MA, USA) equipped with a HESI II probe in polar switching mode with source parameters set as follows: sheath gas flow rate, 60; auxiliary gas flow rate, 17; sweep gas flow rate, 1; spray voltage,+3.5/-3.0 kV; capillary temperature, 275 oC; S-lens RF level, 70; and heater temperature, 325 °C. Data was collected at dd-MS2 mode. Data analysis was performed using Lipidsearch (Thermofisher Scientific/Mitsui Knowledge Industries) with the default parameters for Orbitrap MS Product Search and Alignment. After alignment, raw peak areas for all identified lipids were exported to Excel files. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004579 |
| Analysis ID: | AN004833 |
| Instrument Name: | Thermo Orbitrap Exploris 120 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The mass spectrometry analysis was processed using an Orbitrap Exploris 120 mass spectrometer Thermo Fisher (Waltham, MA, USA) equipped with a HESI II probe in polar switching mode with source parameters set as follows: sheath gas flow rate, 60; auxiliary gas flow rate, 17; sweep gas flow rate, 1; spray voltage,+3.5/-3.0 kV; capillary temperature, 275 oC; S-lens RF level, 70; and heater temperature, 325 °C. Data was collected at dd-MS2 mode. Data analysis was performed using Lipidsearch (Thermofisher Scientific/Mitsui Knowledge Industries) with the default parameters for Orbitrap MS Product Search and Alignment. After alignment, raw peak areas for all identified lipids were exported to Excel files. |
| Ion Mode: | NEGATIVE |
