Return to study ST000095 main page
MB Sample ID: SA005402
| Local Sample ID: | T30-2 |
| Subject ID: | SU000114 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 9 months |
| Gender: | Female |
| Animal Housing: | Singly housed |
| Animal Feed: | High fat diet (HFD, 4.73 kcal/g, 20% protein, 35% carbohydrate, and 45% fat per kcal; D12451, Research Diets, New Brunswick, NJ) |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000110 |
| Sampleprep Summary: | A 15 mg liver sample (n=1 female/litter in 7 VEH and 8 DDT litters) was pulverized on dry ice, enriched with deuterated bile acid surrogates, butylated hydroxytoluene and ethylinediaminetetraacetic acid, and extracted twice with 500 uL methanol. The combined extract was dried, reconstituted in 100uL 50:50 methanol:acetonitrile with internal standards 1-phenyl-3-hexanoic acid urea and 1-cyclohexyl-3-dodecanoic acid urea (Sigma-Aldrich, St. Louis, MO) and filtered at 0.1 µm. |
| Sampleprep Protocol Filename: | Newman_Lab_Bile_Acid_Protocols_La_Merrill_WCMC_P&F.pdf |
| Processing Method: | Pulverization on dry ice |
| Extraction Method: | Methanol |
| Extract Concentration Dilution: | Dried with Rotoevaporator |
| Extract Storage: | Placed in 10 °C autosampler and immediately analyzed |
| Sample Spiking: | Spiked with deuterated bile acid surrogates. |
| Organ: | Liver |
