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MB Sample ID: SA007396

Local Sample ID:SeM (PNT1A)
Subject ID:SU000153
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Biosource Or Supplier:ATCC, Sigma
Cell Strain Details:DU145 prostate cancer, PNT1A prostate nontumor
Subject Comments:10
Cell Primary Immortalized:Immortalized
Cell Passage Number:10
Cell Counts:10
Species Group:Human

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Sample Preparation:

Sampleprep ID:SP000150
Sampleprep Summary:Cells were grown to 80% confluence in Petri dishes (50 mm in diameter) in 4.5% CO2 at 37 OC for 5-6 days for full attachment to the substratum. After aspirating the old growth medium, 3 mL of SeM or SeMSC (300 mM) in fresh pre-warmed medium at 37 ÂșC was added to each dish. After incubation for 24 hrs, cells were washed twice with 0.5 mL PBS in D2O (Cambridge Isotope Laboratories). D2O (0.7 mL) was again added to each petri dish and cells were gently scraped from the surface with a sterile cell scraper. A volume of 0.7 mL of the harvested cell suspension was transferred into a 5 mm NMR tube and 10 mL of 100 mM TMSP in D2O was added to each tube. Prior to NMR analysis, tubes were vortexed to ensure samples were in suspension. Control experiments using untreated cells were conducted in parallel with treated cells with the same incubation protocols and sample preparation.
Sampleprep Protocol Filename:Prostate_Cells_Metabolomics_Procedure.doc
Sample Resuspension:in deuterated water
Cell Type:prostate