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MB Sample ID: SA091493

Local Sample ID:Fn-Sg_1
Subject ID:SU001328
Subject Type:Bacteria
Subject Species:Fusobacterium nucleatum subsp. nucleatum ATCC 25586
Taxonomy ID:190304
Genotype Strain:ATCC 25586
Cell Biosource Or Supplier:ATCC 25586

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Sample Preparation:

Sampleprep ID:SP001336
Sampleprep Summary:To remove protein, 2 ml of chloroform and 0.8 ml of ultrapure water were added to the samples, which were thoroughly mixed and centrifuged at 2300 × g for 5 minutes at 4˚C. The upper aqueous layer was then transferred to ultrafilter tips (Amicon ultrafilter system™) and centrifuged at 9100 × g for 120 minutes at 4˚C. Filtered material was dried under reduced pressure, followed by suspension in 50 µl of ultrapure water.
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