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MB Sample ID: SA091494
Local Sample ID: | Fn-SgVp_1 |
Subject ID: | SU001328 |
Subject Type: | Bacteria |
Subject Species: | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 |
Taxonomy ID: | 190304 |
Genotype Strain: | ATCC 25586 |
Cell Biosource Or Supplier: | ATCC 25586 |
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Sample Preparation:
Sampleprep ID: | SP001336 |
Sampleprep Summary: | To remove protein, 2 ml of chloroform and 0.8 ml of ultrapure water were added to the samples, which were thoroughly mixed and centrifuged at 2300 × g for 5 minutes at 4˚C. The upper aqueous layer was then transferred to ultrafilter tips (Amicon ultrafilter system™) and centrifuged at 9100 × g for 120 minutes at 4˚C. Filtered material was dried under reduced pressure, followed by suspension in 50 µl of ultrapure water. |