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MB Sample ID: SA268847
Local Sample ID: | Fig2 N2 2min KCl |
Subject ID: | SU002803 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
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Sample Preparation:
Sampleprep ID: | SP002809 |
Sampleprep Summary: | At the specific time chosen for thermal preservation, the slices were flash heated to 80°C for 2 seconds and then cooled to 4°C; the slices were then flash-frozen to -20 °C. The brain slices were stored at -80°C for subsequent thin sectioning in a cryomicrotome. The thin sections were mounted onto conductive glass slides (ITO slides) and sprayed with matrix (1,5-diaminonaphthalene). |