Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA318103

Local Sample ID:	HIF KO C2C12 UnT5 M_Dasarathy_Nicole_114Cells_02042022_HILIC_POS253
Subject ID:	SU003043
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090
Age Or Age Range:	Passage 4-8
Gender:	Female
Species Group:	Mammals

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MS

Sample Preparation:

Sampleprep ID:	SP003049
Sampleprep Summary:	Metabolites were extracted from myotubes and skeletal muscle using chilled methanol containing 6 labeled internal standards (Betaine-d9, Carnitine-d9, Estrone-13C3, Cholesterol-13C3, Valeric acid-d9, Choline-d13). After centrifugation at 14,000g for 20 minutes to precipitate the protein pellet, the supernatant was removed, dried, and reconstituted in 5% acetonitrile. LC/MS (liquid chromatography/mass spectrometry) analysis was then performed, including on pooled quality control samples.

Sampleprep ID:

SP003049

Sampleprep Summary:

Metabolites were extracted from myotubes and skeletal muscle using chilled methanol containing 6 labeled internal standards (Betaine-d9, Carnitine-d9, Estrone-13C3, Cholesterol-13C3, Valeric acid-d9, Choline-d13). After centrifugation at 14,000g for 20 minutes to precipitate the protein pellet, the supernatant was removed, dried, and reconstituted in 5% acetonitrile. LC/MS (liquid chromatography/mass spectrometry) analysis was then performed, including on pooled quality control samples.